Staining:Article Title: Intercellular communication atlas reveals Oprm1 as a neuroprotective factor for retinal ganglion cells
Article Snippet: .. The antibodies should be included to verify the findings include goat anti-Osteopontin/Spp1 R&D Systems Cat#AF808; rabbit anti-Melanopsin ATSbio Cat#AB-N38; rabbit anti-Foxp2Abcam Cat#ab16046; rabbit anti-Tbr1 Cell Signaling TechnologyCat#49661; rabbit anti-Satb1 Abcam Cat#ab109122; RESPONSE: To address the reviewer’s concerns, we performed antibody staining with various RGC subclass markers in Oprm1 mCherry tagged mice (see Figures below). ..
Article Title: Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies chicken anti-GFP Abcam Cat#ab13970; RRID:AB_300798 guinea pig anti-RBPMS Phospho Solutions Cat#1832-RBPMS; RRID:AB_2492226 rabbit anti-RBPMS Proteintech Cat#15187-1-AP; RRID:AB_2238431 goat anti-Osteopontin/Spp1 R&D Systems CatAF808; RRID:AB_2194992 rabbit anti-Melanopsin ATSbio Cat#AB-N38; RRID:AB_1608077 rabbit anti-phosphorylated S6 [Ser235/236] Cell Signaling Technology Cat#4856; RRID:AB_2181037 rabbit anti-Foxp2 Abcam Cat#ab16046; RRID:AB_2107107 rabbit anti-Tbr1 Cell Signaling Technology Cat#49661; RRID:AB_2799364 rabbit anti-Satb1 Abcam Cat#ab109122; RRID:AB_10862207 mouse anti-Kv4.2 UC Davis NeuroMab Facility Cat#75-361; RRID:AB_2315874 mouse anti-neurofilament (SMI32) Biolegend Cat#801701; RRID:AB_2564642 mouse anti-GFAP Sigma-Aldrich Cat#G3893; RRID:AB_477010 rabbit anti-Calbindin Swant Cat#CB38; RRID:AB_10000340 rat anti-Integrin alpha V Abcam Cat#ab63490; RRID:AB_1140041 rabbit anti-RFP Rockland Cat#600-401-379; RRID:AB_2209751 NeuroTraceTM 435/455 Blue Fluorescent Nissl Stain Invitrogen Cat#N21479 rat anti-CD44 Millipore Cat#217594; RRID:AB_2076209 mouse anti-Glutamine Synthetase BD Transduction Laboratories Cat#610518; RRID:AB_397880 Bacterial and Virus Strains AAV-EF1a-BbChT Addgene Cat#45186 AAV-EF1a-DIO-Spp1 Boston Children’s Hospital Viral Core N/A AAV-mSncg-Cas9 Stanford Ophthalmology Viral Core Wang et al.46 AAV-sgSpp1-Ef1a-DIO-mOrange2 This study N/A AAV-sgRNA-non-targeting Ef1a-DIOmOrange2 This study N/A Biological Samples Mouse eye tissues Mouse strain is listed in the " Experimental Models: Organisms/Strains." .. N/A Human adult eye retinal tissues Research Autopsy Collaboration (RACS) at Stanford University IRB#63818 Human prenatal eye retinal tissues Human Gamete, Embryo, and Stem Cell Research Committee at the University of California, San Francisco IRB#10-05113 Human aqueous humor fluid University of California San Francisco, Department of Ophthalmology IRB#17-22840 Chemicals, Peptides, and Recombinant Proteins Silicone oil Alcon Laboratories 1,000 mPa s, Silikon Experimental Models: Organisms/Strains Mouse: C57BL/6J (WT) Jackson Laboratory Cat# 000664 Mouse: Kcng4-Cre Jackson Laboratory Cat# 029414 Mouse: Foxp2-Cre Jackson Laboratory Cat# 030541 Mouse: Opn4-Cre Jackson Laboratory Cat# 035925 Mouse: Cartpt-IRES-Cre Jackson Laboratory Cat# 028533 Mouse: TWY3 Jackson Laboratory Cat# 033114 (Continued on next page) 12 Cell Reports 42, 113038, September 26, 2023
Virus:Article Title: Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies chicken anti-GFP Abcam Cat#ab13970; RRID:AB_300798 guinea pig anti-RBPMS Phospho Solutions Cat#1832-RBPMS; RRID:AB_2492226 rabbit anti-RBPMS Proteintech Cat#15187-1-AP; RRID:AB_2238431 goat anti-Osteopontin/Spp1 R&D Systems CatAF808; RRID:AB_2194992 rabbit anti-Melanopsin ATSbio Cat#AB-N38; RRID:AB_1608077 rabbit anti-phosphorylated S6 [Ser235/236] Cell Signaling Technology Cat#4856; RRID:AB_2181037 rabbit anti-Foxp2 Abcam Cat#ab16046; RRID:AB_2107107 rabbit anti-Tbr1 Cell Signaling Technology Cat#49661; RRID:AB_2799364 rabbit anti-Satb1 Abcam Cat#ab109122; RRID:AB_10862207 mouse anti-Kv4.2 UC Davis NeuroMab Facility Cat#75-361; RRID:AB_2315874 mouse anti-neurofilament (SMI32) Biolegend Cat#801701; RRID:AB_2564642 mouse anti-GFAP Sigma-Aldrich Cat#G3893; RRID:AB_477010 rabbit anti-Calbindin Swant Cat#CB38; RRID:AB_10000340 rat anti-Integrin alpha V Abcam Cat#ab63490; RRID:AB_1140041 rabbit anti-RFP Rockland Cat#600-401-379; RRID:AB_2209751 NeuroTraceTM 435/455 Blue Fluorescent Nissl Stain Invitrogen Cat#N21479 rat anti-CD44 Millipore Cat#217594; RRID:AB_2076209 mouse anti-Glutamine Synthetase BD Transduction Laboratories Cat#610518; RRID:AB_397880 Bacterial and Virus Strains AAV-EF1a-BbChT Addgene Cat#45186 AAV-EF1a-DIO-Spp1 Boston Children’s Hospital Viral Core N/A AAV-mSncg-Cas9 Stanford Ophthalmology Viral Core Wang et al.46 AAV-sgSpp1-Ef1a-DIO-mOrange2 This study N/A AAV-sgRNA-non-targeting Ef1a-DIOmOrange2 This study N/A Biological Samples Mouse eye tissues Mouse strain is listed in the " Experimental Models: Organisms/Strains." .. N/A Human adult eye retinal tissues Research Autopsy Collaboration (RACS) at Stanford University IRB#63818 Human prenatal eye retinal tissues Human Gamete, Embryo, and Stem Cell Research Committee at the University of California, San Francisco IRB#10-05113 Human aqueous humor fluid University of California San Francisco, Department of Ophthalmology IRB#17-22840 Chemicals, Peptides, and Recombinant Proteins Silicone oil Alcon Laboratories 1,000 mPa s, Silikon Experimental Models: Organisms/Strains Mouse: C57BL/6J (WT) Jackson Laboratory Cat# 000664 Mouse: Kcng4-Cre Jackson Laboratory Cat# 029414 Mouse: Foxp2-Cre Jackson Laboratory Cat# 030541 Mouse: Opn4-Cre Jackson Laboratory Cat# 035925 Mouse: Cartpt-IRES-Cre Jackson Laboratory Cat# 028533 Mouse: TWY3 Jackson Laboratory Cat# 033114 (Continued on next page) 12 Cell Reports 42, 113038, September 26, 2023
Bioprocessing:Article Title: Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies chicken anti-GFP Abcam Cat#ab13970; RRID:AB_300798 guinea pig anti-RBPMS Phospho Solutions Cat#1832-RBPMS; RRID:AB_2492226 rabbit anti-RBPMS Proteintech Cat#15187-1-AP; RRID:AB_2238431 goat anti-Osteopontin/Spp1 R&D Systems CatAF808; RRID:AB_2194992 rabbit anti-Melanopsin ATSbio Cat#AB-N38; RRID:AB_1608077 rabbit anti-phosphorylated S6 [Ser235/236] Cell Signaling Technology Cat#4856; RRID:AB_2181037 rabbit anti-Foxp2 Abcam Cat#ab16046; RRID:AB_2107107 rabbit anti-Tbr1 Cell Signaling Technology Cat#49661; RRID:AB_2799364 rabbit anti-Satb1 Abcam Cat#ab109122; RRID:AB_10862207 mouse anti-Kv4.2 UC Davis NeuroMab Facility Cat#75-361; RRID:AB_2315874 mouse anti-neurofilament (SMI32) Biolegend Cat#801701; RRID:AB_2564642 mouse anti-GFAP Sigma-Aldrich Cat#G3893; RRID:AB_477010 rabbit anti-Calbindin Swant Cat#CB38; RRID:AB_10000340 rat anti-Integrin alpha V Abcam Cat#ab63490; RRID:AB_1140041 rabbit anti-RFP Rockland Cat#600-401-379; RRID:AB_2209751 NeuroTraceTM 435/455 Blue Fluorescent Nissl Stain Invitrogen Cat#N21479 rat anti-CD44 Millipore Cat#217594; RRID:AB_2076209 mouse anti-Glutamine Synthetase BD Transduction Laboratories Cat#610518; RRID:AB_397880 Bacterial and Virus Strains AAV-EF1a-BbChT Addgene Cat#45186 AAV-EF1a-DIO-Spp1 Boston Children’s Hospital Viral Core N/A AAV-mSncg-Cas9 Stanford Ophthalmology Viral Core Wang et al.46 AAV-sgSpp1-Ef1a-DIO-mOrange2 This study N/A AAV-sgRNA-non-targeting Ef1a-DIOmOrange2 This study N/A Biological Samples Mouse eye tissues Mouse strain is listed in the " Experimental Models: Organisms/Strains." .. N/A Human adult eye retinal tissues Research Autopsy Collaboration (RACS) at Stanford University IRB#63818 Human prenatal eye retinal tissues Human Gamete, Embryo, and Stem Cell Research Committee at the University of California, San Francisco IRB#10-05113 Human aqueous humor fluid University of California San Francisco, Department of Ophthalmology IRB#17-22840 Chemicals, Peptides, and Recombinant Proteins Silicone oil Alcon Laboratories 1,000 mPa s, Silikon Experimental Models: Organisms/Strains Mouse: C57BL/6J (WT) Jackson Laboratory Cat# 000664 Mouse: Kcng4-Cre Jackson Laboratory Cat# 029414 Mouse: Foxp2-Cre Jackson Laboratory Cat# 030541 Mouse: Opn4-Cre Jackson Laboratory Cat# 035925 Mouse: Cartpt-IRES-Cre Jackson Laboratory Cat# 028533 Mouse: TWY3 Jackson Laboratory Cat# 033114 (Continued on next page) 12 Cell Reports 42, 113038, September 26, 2023
Recombinant:
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